9/22/2023 0 Comments 5 minute journal free downloadDNA origami patterning of synthetic T cell receptors reveals spatial control of the sensitivity and kinetics of signal activation. Nanoscale FasL organization on DNA origami to decipher apoptosis signal activation in cells. Nanomechanical DNA origami ‘single-molecule beacons’ directly imaged by atomic force microscopy. Kuzuya, A., Sakai, Y., Yamazaki, T., Xu, Y. Rotation tracking of genome-processing enzymes using DNA origami rotors. A DNA origami-based chiral plasmonic sensing device. Facile and label-free electrochemical biosensors for microRNA detection based on DNA origami nanostructures. Role of nanoscale antigen organization on B-cell activation probed using DNA origami. A DNA nanodevice-based vaccine for cancer immunotherapy. DNA origami as an in vivo drug delivery vehicle for cancer therapy. A DNA nanorobot functions as a cancer therapeutic in response to a molecular trigger in vivo. A logic-gated nanorobot for targeted transport of molecular payloads. Engineering DNA nanostructures to manipulate immune receptor signaling and immune cell fates. Folding DNA to create nanoscale shapes and patterns. DNA origami: scaffolds for creating higher order structures. Together, origamiFISH enables the accurate mapping of DN distribution across subcellular and tissue barriers for guiding the development of DN-based therapeutics.ĭey, S. We additionally optimize compatibility with immunofluorescence and tissue clearing to visualize DN distribution within tissue cryo-/vibratome sections, slice cultures and whole-mount organoids. We identify cell-type- and DN shape-specific spatiotemporal distribution patterns within a minute of uptake and at picomolar DN concentrations, 10,000× lower than field standards. origamiFISH targets pan-DN scaffold sequences with hybridization chain reaction probes to achieve 1,000-fold signal amplification. Here we present origamiFISH, a label-free and universal method for the single-molecule fluorescence detection of DNA origami nanostructures in cells and tissues. Current methods for tracking DN fates in situ, including fluorescent-dye labelling, suffer from low sensitivity and dye-induced artifacts. However, the role of DN design during cellular interactions and subsequent biodistribution remain poorly understood. Structural DNA nanotechnology enables the fabrication of user-defined DNA origami nanostructures (DNs) for biological applications.
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